Extended Data Fig. 2: QC metrics for scRNA-seq data – vSMC only analysis.
From: Region-specific gene expression and sex inform about disease susceptibility in the aorta
a-c, Violin plots representing number of genes detected in each cell (nFeature_RNA) (a), total number of RNA molecules detected within a cell (nCount_RNA) (b), and percentage of transcript counts arising from the mitochondrial genome (percent.mito) (c) per each anatomic location. d-f, Violin plots representing number of genes detected in each cell (nFeature_RNA) (d), total number of RNA molecules detected within a cell (nCount_RNA) (e), and percentage of transcript counts arising from the mitochondrial genome (percent.mito) (f), for each individual cell cluster identified by sex and anatomic site. g, Bar plot visualizing the number of vSMCs analyzed by anatomic location. h, Bar plot visualizing the number of vSMCs analyzed by anatomic location and biological sex. i, Stacked barplot visualizing the contribution of vSMCs by sex. j, Venn diagram visualizing the overlap between carotid vSMC anatomic signature, female-enriched carotid vSMC genes and male- enriched carotid vSMC signature. k, Venn diagram visualizing the overlap between the aortic arch vSMC anatomic gene signature, female-enriched aortic arch vSMC genes and male- enriched aortic arch vSMC genes. l, Venn diagram visualizing the overlap between the thoracic aorta vSMC anatomic signature, female-enriched thoracic vSMC genes and male - enriched thoracic vSMC signature. m, Venn diagram visualizing the overlap between the abdominal aorta vSMC anatomic signature, female-enriched abdominal aorta vSMC genes and male- enriched abdominal vSMC signature
