Extended Data Fig. 4: Mcam in health and disease.
From: Region-specific gene expression and sex inform about disease susceptibility in the aorta
a, Immunofluorescent staining of aortic rings taken from control and Mcam−/− animals stained with MCAM (red) and alpha-smooth muscle actin (aSMA, green). b, Representative IF images of MCAM expression (green) in female and male thoracic vSMCs. c, Quantification of MCAM intensity in female and male vSMCs. Females n = 30 images, 5 animals and males n = 17 images, 5 animals, 47 images total. Error bars represent mean ± SD. Two-tailed unpaired t-test with Welch’s correction. p < 0.0001 d, Violin plot visualizing the expression pattern of Mcam in thoracic vSMCs by sex. e-g, Violin plots representing: (e) number of genes detected in each cell (nFeature_RNA), (f) total number of RNA molecules detected within a cell (nCount_RNA), (g) and percentage of transcript counts arising from the mitochondrial genome (percent.mito) in control and AngII-driven abdominal aortic aneurysm (AAA) model. h-j, Violin plots representing: (h) number of genes detected in each cell (nFeature_RNA), (i) total number of RNA molecules detected within a cell (nCount_RNA), and (j) percentage of transcript counts arising from the mitochondrial genome (percent.mito) for each individual cell type in the AAA dataset using Seurat. k-m, Violin plots representing: (k) number of genes detected in each cell (nFeature_RNA), (l) total number of RNA molecules detected within a cell (nCount_RNA) (m), and percentage of transcript counts arising from the mitochondrial genome (percent.mito) in control and elastase-treatment induced AAA model. n-p, Violin plots representing: (n) number of genes detected in each cell (nFeature_RNA), (o) total number of RNA molecules detected within a cell (nCount_RNA), (p) and percentage of transcript counts arising from the mitochondrial genome (percent.mito) for each individual cell type in the elastase dataset using Seurat. q, UMAP plot visualizing cell distribution by celltype and dataset in control or elastase-treated aortas. r, Violin plot visualizing the expression of Mcam in control or elastase treated aortas. Data e-g represents a reanalysis of Hadi 2019, whereas Data k-r represents a reanalysis of Zhao33. Abbreviations, EC - endothelial cell, vSMC - vascular smooth muscle cell.
