Extended Data Fig. 8: Network analysis showing LEC-macrophage signalling.
From: Cardiac lymphatics retain LYVE-1-dependent macrophages during neonatal mouse heart regeneration
Tree plot showing outgoing connections from the LECs to Mf (A) and from Mf to LECs (B). The single outgoing LEC signal was identified as Reelin (Reln), a previously described lymphangiocrine factor, interacting with ITGβ▢ on Mf (A). Top node refers to source population, second layer to ligands, third layer to receptors and bottom node representing the target population (A, B). Reln expression was shown by qPCR to decrease between P1 (n = 6) and P7 (n = 5), while Itgb1 expression was unchanged (C). Immunostaining for ITGFB1 combined with IBA1 (D) and REELIN combined with LYVE-1 (E-H) in resident macrophages and LECs, respectively, revealed elevated expression of Reelin at P2MI5dpi compared to P7MI5dpi and sham controls across equivalent timepoints (Figure E-H). Data are presented as mean ± SD. Qualitative observations in D-H were seen across tissue sections and in 1 additional heart. Scale bar: 25 μm for D; 150 μm for E-H.
