Extended Data Fig. 10: hCD68-CreERT2;R26R-TdTomato reports LYVE-1 positive macrophages in P2 hearts 7 days post-MI and MRI cine images of hCD68-CreERT2;Lyve1fl°x/fl°x hearts 28-days post-MI with corresponding picrosirius red staining.
From: Cardiac lymphatics retain LYVE-1-dependent macrophages during neonatal mouse heart regeneration
Macrophages expressing TdTomato in P2 infarcted hearts at 7dpi. LYVE-1 + /TdTomato+ macrophages confirmed by co-staining with CD68 and LYVE-1 (A). Quantification of co-staining in sections (n = 3) (B). Tamoxifen injected at P1, MI surgery at P2 to avoid inducing respiratory failure. Longitudinal and transverse cine MRI representative images from control and hCD68-CreERT2+ animals at P2MI28dpi accompanied by transverse picrosirius sections matched to the corresponding level and illustrated by schematic (C, D). Picrosirius red staining revealed no significant difference in relative scar area between control and hCD68-CreERT2+ animals at 28 days post-MI (E). Asterisk marks suture site. RA = right atrium, LA = left atrium, RV = right ventricle, LV = left ventricle. n = 7 for control, n = 13 for hCD68CreERT2;Lyve1fl/fl hearts. Panel C created in BioRender; Chapman, B. (2025) https://BioRender.com/z1nstjj. Unpaired Student’s t test was used to determine significance. Data are presented as mean ± SD. In A, scale bar 350μm; zoomed 150μm. In D, scale bar 5 mm in MRI; 1 mm in picrosirius sections.
