Fig. 7: Functional MRI parameters of P1 versus P7 Lyve1 KO and macrophage-specific Lyve1 KO hearts 28 days after MI.
From: Cardiac lymphatics retain LYVE-1-dependent macrophages during neonatal mouse heart regeneration
Plots from longitudinal cine MRI performed on Lyve1+/− and Lyve1−/− mice 28 days after MI at P1 and P7 as well as in intact control littermates at comparable P29 and P35 stages (a,b). MRI revealed significantly reduced cardiac output in P1 Lyve1−/− mice at 28 days after MI compared to P29 intact Lyve1−/− controls (a). The reduced cardiac output was even more significant than reductions observed for Lyve1−/− mice injured at P7 at 28 days after MI compared to P35 intact Lyve1−/− controls (b). Plots from MRI performed on hCD68–CreERT2;Lyve1flox/flox mice 28 days after MI at P2 reveal impaired functional recovery across cardiac output, StV, ejection fraction and end diastolic volume consistent with a, differing only in end systolic volume (c). Data are presented as mean ± s.d. n = 6 for P29 control, n = 7 for P1MI28dpi control, n = 8 for P29 Lyve1−/−, n = 7 for P1MI28dpi Lyve1−/−, n = 8 for P35 control, n = 7 for P7MI28dpi control, n = 8 for P35 Lyve1−/−, n = 10 for P7MI28dpi Lyve1−/−, n = 7 for P2MI28dpi, n = 13 for P2MI28dpi. Significant differences were calculated using two-way ANOVA for a and b and unpaired two-tailed Student’s t-test for c.
