Fig. 1: The majority of aortic IFNγ+ T cells in hypercholesteremic mice are PD-1+.
a,b, IFNγ–YFP reporter mice were crossed onto an atherosclerotic background (Apoe−/−) and fed an HCD for 12 weeks, and flow cytometry was performed to characterize IFNγ-producing T cells (n = 11). c–e, Comparison of PD-1 expression between IFNγ–YFP− and IFNγ–YFP+ aortic CD4 (****P = 1.8 × 10−6, 1.2 × 10−5) and CD8 T cells (****P = 5.7 × 10−6, 9.9 × 10−5). f,g, Comparison of PD-1 expression on CD4 (**P = 0.0068, ***P = 0.0002) and CD8 (**P = 0.0045, 0.0021, 0.0039, respectively) T cells between spleen, aortic-draining iliac lymph node (aLN), blood and aorta. h, PD-1 expression comparing antigen-experienced CD44+ IFNγ–YFP+/− T cells. i, scRNA-seq analysis of differential gene expression comparing aortic antigen-experienced (CD44-expressing) T cells with or without PD-1 expression. d, Bars denote mean, analyzed with two-sided unpaired t-test. e, Bars denote median, analyzed with two-sided Mann–Whitney U-test. f,g, Bars denote mean, analyzed with two-sided repeated-measures one-way ANOVA test compared to aorta. i, Adjusted Pvalue after FDR adjustment. P values are reported top-to-bottom and left-to-right. FC, fold change.
