Extended Data Fig. 2: Comparative analysis of aortic and tumor-infiltrating exhausted T cells. | Nature Cardiovascular Research

Extended Data Fig. 2: Comparative analysis of aortic and tumor-infiltrating exhausted T cells.

From: Progenitor exhausted PD-1+ T cells are cellular targets of immune checkpoint inhibition in atherosclerosis

Extended Data Fig. 2

IfngYFP/YFPApoe−/− mice (n = 7) were fed a high-cholesterol diet and injected with MC38 tumor cells. Aortas and tumors were harvested 2 weeks after tumor implantation. T cells were stratified based on the degree of cell-surface PD1 expression: CD44+PD1− (PD1−), CD44+PD1intermediate (PD1int), and CD44+PD1high (PD1high). (a) Tumor growth over time. Data presented as mean values +/- SD. (b) H&E staining of MC38 tumor (d14). (c) Flow cytometry gating strategy for identifying T cell PD1 subsets. (d-e) Frequency of PD1 CD4 and CD8 subsets of T cells in the aorta and tumor. (f-g) IFN-γ production by each PD1 T cell subset in the aorta and tumor. (d-g) Bars denote median, analyzed with two-sided Wilcoxon matched-pairs test (*p = 0.016).

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