Extended Data Fig. 4: PD1 expression in relation to T-cell receptor signaling and IL-2 signaling. | Nature Cardiovascular Research

Extended Data Fig. 4: PD1 expression in relation to T-cell receptor signaling and IL-2 signaling.

From: Progenitor exhausted PD-1+ T cells are cellular targets of immune checkpoint inhibition in atherosclerosis

Extended Data Fig. 4

(a-c) Atherosclerotic dual-reporter mice (IfngYFP/YFPNur77wt/GFPApoe−/−) were generated, fed a high-cholesterol diet for 10 weeks, and flow cytometry was performed to characterize Nur77 expression on IFN-γ-producing T cells in spleen, iliac aortic-draining lymph node (aLN), and aorta (n = 6). (a) Flow cytometry plots and (b-c) quantification of association between Nur77-GFP expression and PD1 expression within IFN-γ-YFP+ CD4 and CD8 T cells in the aorta, iliac aortic-draining lymph nodes (aLN), and spleen. (d) Representative flow cytometry gating for identifying follicular T helper cells (Tfh; CXC5+PD1high CD4+) in IfngYFP/YFPApoe−/− mice. (e) Experimental design for anti-IL-2 study. IfngYFP/YFPApoe−/− mice were fed a high cholesterol diet (HCD) for 10 weeks before receiving i.p. injections (3 doses, 5 days apart) of anti-IL-2 antibodies or control IgG (n = 9/group) for 2 weeks.

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