Extended Data Fig. 5: Exhaustion phenotype of ApoB-100-specific transgenic T cells.

Transgenic and congenic (CD45.1) CD4 T cells (BT3) reactive against a peptide sequence of human apolipoprotein B-100 (apoB-100; TRBV31⁺ CD4 T cells) were transferred into atherosclerotic mice that produce human apoB-100 (APOB100^TgLdlr^−/−; HuBL mouse). HuBL mice were fed a high-cholesterol diet for three weeks after adoptive transfer and then terminated (n = 6/group). (a) Gating strategy identifying CD44⁺ host CD4 and CD45.1⁺ BT3 T cells. (b-c) Quantification of PD1 subset composition comparing host CD4 and BT3 T cells in spleen (**p = 0.0022) and aortic-draining iliac lymph node (aLN, **p = 0.0022). Frequencies of (d) CD44⁺Tim3⁺ CD4 T cells and (e) CD44⁺Tox⁺ CD4 T cells within host and BT3 CD4 T cells in aLN (**p = 0.0079). (b-e) Bars denote median, analyzed with two-sided Mann-Whitney U test.

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