Fig. 3: Aortic PD-1-expressing T cells are polyfunctional. | Nature Cardiovascular Research

Fig. 3: Aortic PD-1-expressing T cells are polyfunctional.

From: Progenitor exhausted PD-1+ T cells are cellular targets of immune checkpoint inhibition in atherosclerosis

Fig. 3

a,b, Aortas from IfngYFP/YFPApoe−/− mice were digested and pooled (n = 2 per pool, eight pools total). Representative flow cytometry and quantification of high Ki67 expression within CD44+PD-1 subsets of aortic CD4 (****P = 1.9 × 10−5, **P = 0.0014, *P = 0.033) and CD8 (***P = 0.0001, **P = 0.0013) T cells. c–f, Digested and pooled aortas (n = 3–4 per pool, three pools total) were stimulated ex vivo with PMA/ionomycin/brefeldin A for 4 hours, followed by flow cytometry. Each PD-1 subset within CD4 and CD8 T cells was analyzed for cytokine production and co-production of IFNγ (measured here with anti-IFNγ-APC antibody), TNF and IL-2. a,b, Bars denote median, analyzed with two-sided Kruskal–Wallis test.

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