Fig. 4: T8Ab treatment following MI promotes a cardioprotective gene signature.
From: ANTXR1 blockade enhances cardiac function in preclinical models of heart failure
a, Uniform Manifold Approximation and Projection (UMAP) display of cardiac cell subpopulations. The diminished blood EC (BEC) population at MI d7 is highlighted (blue circle). b, Trajectory analysis of the six CF subclusters. c, Violin plot showing Antxr1 and Postn expression in each annotated cell type. d, Violin plot showing the total Antxr1 mRNA expression in CFs from MI d7/14 versus sham. e, Bar graph showing % increase of Antxr1-positive CF4–6 cells after MI d7/14 versus sham. n = 3 per group (sham) or 2 per group (MI samples), with each sample derived by pooling two hearts to minimize variability. f, GO analysis comparing pathway activation in CF1–3 versus CF4–6 at MI d7. Reg., regulation. g, Co-IF staining for ANTXR1 (green), THBS4 (red) and TNNT2 (magenta) in LV on day 7 post-MI. Image is representative of at least four hearts. Scale bar, 200 µm. h,i, Heat maps showing gene expression changes in the MI d7 + T8Ab and MI d7 + vehicle groups compared to sham (h) and the MI d14 + T8Ab and MI d14 + vehicle groups compared to sham (i). j,k, Violin plots showing the average expression of all genes upregulated by T8Ab at MI d7 (j) and downregulated by T8Ab at MI d14 (k) in h and i, respectively. inc., increased; dec., decreased. l,m, Violin plots showing Postn (l) and Meox1 (m) expression. n, qRT–PCR validation of Meox1 mRNA expression. n = 3 per group (sham) or 2 per group (MI samples), with each sample derived by pooling two hearts to minimize variability. Data are shown as mean ± s.e.m. One-way ANOVA (RT–PCR) with Tukey’s multiple-comparisons test (e,n) or two-tailed Wilcoxon test (violin plots) (d,j,k–m). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NS, nonsignificant.
