Extended Data Fig. 1: Localization of ANTXR1 post-myocardial infarction.
From: ANTXR1 blockade enhances cardiac function in preclinical models of heart failure
a) Immunofluorescence staining was performed on border zone (region between scar and remote area) of a left ventricle (LV) sample taken from the failing heart of a 55-year-old transplant patient ~2.5 months following myocardial infarction. Image is representative of 3 independent samples. Note that ANTXR1 (green) was generally enriched near regions with high levels of denatured collagen, as detected with a collagen hybridizing peptide (CHP, orange). Anti-TNNI3 (cardiac troponin) antibodies were used to label cardiomyocytes (red). Bar: 2 mm. b) Localization of ANTXR1 in mouse hearts was examined by performing immunofluorescence staining for ANTXR1 (green) and TNNT2 (red) on hearts taken at various time points following myocardial infarction (MI). Images are representative of at least 3 independent samples per time point. Sham controls (surgery without MI) and MI hearts taken from Antxr1 KO mice were both taken at d7 post-surgery and used to demonstrate specificity. Bar: 1 mm.
