Fig. 2: Relationship between motion speed and iPSC-PLTs yield.
From: Defective flow space limits the scaling up of turbulence bioreactors for platelet generation
a Representative flow cytometry dot plots of CD41 (X-axis) and CD42b (GPIbα) (Y-axis) from VerMES50 set at 200, 300, and 400 mm s-1. b Production of CD41+CD42b+ iPSC-PLTs from imMKCLs was evaluated in VerMES3: Blue bar (N = 4), VerMES10: Red bar (N = 3), and VerMES50 at 200: Green bar (N = 3), 300: Purple bar (N = 5), or 400 mm s-1: Orange bar (N = 3). Values were taken at Dox-OFF day 6 (mean ± SD). c–e CD62P (P-selectin) (c), PAC-1 binding (d), and Annexin V binding (e) were quantified by flow cytometry. CD62P expression and PAC-1 binding were measured before and after stimulation with 40 μM TRAP and 100 μM ADP. Annexin V binding was evaluated in the presence or absence of 20 μM ionomycin. PLTs platelets, imMKCL immortalized megakaryocyte cell line.
