Fig. 3: Functional assessment in vitro and in vivo of VerMES50 iPSC-PLTs.
From: Defective flow space limits the scaling up of turbulence bioreactors for platelet generation
Evaluations were done after the depletion of remaining imMKCLs and subsequent washing of iPSC-PLTs. a Aggregation of donor PLTs or iPSC-PLTs post agonist stimulation (10 μg/mL collagen or 40 μg/mL TRAP) based on light transmission. Donor PLTs: black line, VerMES50 iPSC-PLTs: pink line. b Bleeding times after transfusion were measured in a thrombocytopenia mouse model. Vehicle (bicarbonated Ringer’s solution with 10% ACD-A and 2.5% albumin, black dots), donor PLTs (white dots), and iPSC-PLTs (3 L: blue triangle, 50L-200 mm s-1: green diamonds, 50L-300 mm s-1: purple diamonds) were transfused at a dose of 2 × 10^8 per mouse. The tail puncture and measurements of bleeding time were performed 2 h after the transfusion. Lines show medians. *P < 0.05, **P < 0.01, Mann-Whitney test vs. vehicle. c Post transfusion kinetics in the thrombocytopenia mouse model. Vehicle (bicarbonated Ringer’s solution with 10% ACD-A and 2.5% albumin, black dots), donor PLTs (white dots), and iPSC-PLTs (3 L: grey triangles, 50 L: pink diamonds) were transfused at a dose of 2 × 10^8 per mouse. Blood sampling was done 0.5, 1, 2, 4, 6, and 24 h after the transfusion. PLTs platelets, iPSC-PLTs induced-pluripotent-stem-cell-derived platelets.
