Fig. 1: Overview of the development and validation of trehalose as a successful cryoprotectant agent (CPA) for mammalian cells.
From: Mesenchymal stem cell cryopreservation with cavitation-mediated trehalose treatment

Trehalose delivered via US + microbubbles enable cryopreservation of at least 4 weeks while preserving cell viability, membrane integrity, and cell multipotency.