Fig. 4: Trehalose-treated MSCs at 3- and 5-days post-treatment and before cryopreservation.
From: Mesenchymal stem cell cryopreservation with cavitation-mediated trehalose treatment
Cells were treated with a range of trehalose concentrations (0–750 mM) and subjected to ultrasonication for either a 1.5 or b 5.0 min at 0.25 MPa. Data are presented as the relative cell viability against the control (cells) on day 3. c MSCs were also imaged at these two timepoints and stained with a live/dead assay to depict live (green) and dead (red) cells. Here, cells treated with 5 min UMT are shown. Control (cells): cells not treated with trehalose and not exposed to ultrasonication, and control (cells + US): cells exposed to ultrasonication but not treated with trehalose. Scale bar: 100 μm. Statistically significant differences (*) in comparison with the control (cells) are shown (two-way ANOVA with Bonferroni’s post hoc test, p < 0.05). Error bars indicate standard deviation (three independent runs; total n = 11).
