Fig. 6: Cell pluripotency post-cryopreservation depicting sample images of cells treated with a range of trehalose concentrations (0–750 mM) delivered via UMT alongside controls.
From: Mesenchymal stem cell cryopreservation with cavitation-mediated trehalose treatment
A control of MSCs not exposed to ultrasonication nor treated with trehalose (Control (cells)) and a control of cells only exposed to ultrasonication (0.25 MPa, 5.0 min; control (cells + US)) but not treated with trehalose were used. Controls were cryopreserved in 10% DSMO + 90% FBS to show the difference between DMSO and trehalose as CPA. During the differentiation assay, positive control cells are treated with the differentiation medium while negative controls are given normal medium: DMEM + 10% FBS. Scale bars: 200 μm (adipogenesis), 100 μm (osteogenesis), or 100 μm (chondrogenesis).
