Fig. 4: Three-dimensional imaging of DiD-stained HeLa cell membranes with 3D-DyFI and piezo stage-based confocal microscopy.
From: Three-dimensional random-access confocal microscopy with 3D remote focusing system
a 3D live cell imaging with piezo stage-based confocal microscopy. b 3D live cell imaging with 3D-DyFI. The corresponding section images in the x-y, x-z, and y-z planes are shown in the right panel. The x-y plane section images were taken at the axial position of z = 0.4 μm; the x-z plane section images were taken at the position of y = 15 μm; and the y-z plane section images were taken at the position of x = 24 μm. Dashed lines at x = 24 μm (cyan) and y = 15 μm (yellow) are marked on the x-y plane image. The color indicates fluorescence intensity in (a) and (b). The intensity profiles along the y = 15 μm (c) and x = 24 μm lines (d), respectively. The relative root means squared errors (RRMSE) between the two intensity profiles were calculated, resulting in 0.031 and 0.082, respectively. Pixel size in x and y: 0.2 μm; Pixel size in z: 0.4 μm.
