Fig. 6: 3D Random-access imaging with 3D-DyFI and piezo stage-based confocal system.
From: Three-dimensional random-access confocal microscopy with 3D remote focusing system
a Three-dimensional image of three fluorescent beads embedded in agarose obtained with 3D-DyFI. The particles are labeled with A, B, and C, respectively. b The segmentary intensity trace of the three fluorescent beads in (a). The illumination time in each period is 2 ms. The lines in different colors indicate different particles as labeled in (a). The continuous intensity trace from three fluorescent beads with 2 ms illumination time (c) and 5 ms illumination (d) in each imaging period. The magenta lines show the z position of laser focus, obtained from the input of 3D dynamics focusing system. The fluorescence intensity is bin in 400 μs, considered the about 800 μs response time of 3D dynamic focusing system. e Three-dimensional image of three fluorescent beads embedded in agarose obtained with piezo stage-based confocal system. The particles are labeled with A, B, and C, respectively. f The segmentary intensity trace of the three fluorescent beads in (e). The illumination time in each period is 50 ms. The lines in different colors indicate different particles as labeled in (e). The continuous intensity trace from three fluorescent beads with 10 ms illumination time (g) and 50 ms illumination (h) in each imaging period. The magenta lines show the readout position from the piezoelectric stage controller. The yellow line indicates the input position of the piezoelectric stage controller. The color indicates fluorescence intensity in (a) and (e).
