Fig. 1: Setup overview and selection of components. | Communications Engineering

Fig. 1: Setup overview and selection of components.

From: Fully automated multicolour structured illumination module for super-resolution microscopy with two excitation colours

Fig. 1

A the complete openSIMMO (open-source structured illumination microscopy module) setup attached to a Nikon Ti2-A Eclipse. B The beam path of the two lasers is folded to minimize the footprint and meet the blaze condition. C Aside from the laser-cut housing and a minimal number of external optics components, the mechanical design relies on the RailOptics system that enables translation of optics along the optical axis. D the digital mirror device (DMD) module is positioned with a customized optomechanical mount and driven via the HDMI port of a Raspberry Pi. E off-the-shelf optics are adjusted using 3D printed adapters mounted on a laser-cut base plate.

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