Fig. 4: Comparing widefiled and structured illumination microscopy (SIM) images of multi-color excitation. | Communications Engineering

Fig. 4: Comparing widefiled and structured illumination microscopy (SIM) images of multi-color excitation.

From: Fully automated multicolour structured illumination module for super-resolution microscopy with two excitation colours

Fig. 4

A SIM Reconstruction and pseudo widefield comparison of Actin (488nm) and Mitochondria (635nm). The sample is GATTA-Cells 4C with stained huFIB cells (F36924, ThermoFisher Scientific, Germany). The structured illumination microscopy (SIM) reconstruction clearly reduces the background and improves the resolution depicted in the line profile E. B The comparison shows how SIM recovers the filamentous structure of actin in AF488 (Phalloidin) labeled samples zoomed in C/D. The sample is FluoCells prepared slide with stained BPAE cells (F36924, ThermoFisher Scientific, Germany). E Line plot showing the pseudo widefield image and SIM reconstruction of mitochondria from A along the magenta line. F Line plot showing the actin filaments from B along the magenta line. The SIM reconstruction separates previously unresolved filament structures.

Back to article page