Fig. 1: Targets of miR-124-3p used in process gene list (PGL) identify the globus pallidus (GP) as the top ROI.

A Prados et al. described the miR-124-3p promoter region as hypomethylated in BPD. We isolated predicted messenger-RNA (mRNA) targets of miR-124-3p from TargetScan v 8.0 and refined the list based on the conserved binding sites in the 3′-utranslated regions (genes with an Aggregate Pct score of >0.99). B, C These 93 genes were utilized for PGL and a list of regions of interest were generated (regions with significant co-expression of the identified genes). We identified a list of regions, including the globus pallidus, external segment left, and internal segment left, as top regions of interest for further study. In each schematic of the brain representing the regions of interest identified, the color-coded points represent the regions as color-coded on the corresponding list below. Ranking: the importance of the ROI (see Supplementary Figs. 1, 2). Freq frequency, the number of times that the list of genes “points” to a certain region in the 6 brains available in the Allen Brain Atlas, only regions that appear as significant in five or six of the brains were used.