Fig. 1: Loss of CpxA renders Yersinia pseudotuberculosis more susceptible to colistin which can be rescued via complementation.

A Minimal inhibitory concentrations (MIC) to colistin of parental (WT), ΔcpxA, ΔcpxA/pWKS_cpxA, and ΔcpxA/pWKS strains. Representative of at least three separate experiments. B 1-h colistin time-kill assay plotted as colony forming units (CFU) shows enhanced killing of ΔcpxA and ΔcpxA/pWKS strains compared to parental (WT) or ΔcpxA/pWKS_cpxA. The difference between parental (WT) or complemented (ΔcpxA/pWKS_cpxA) against ΔcpxA or ΔcpxA/pWKS is statistically significant (for example concentration 0.5, P < 0.05, two-way ANOVA Tukey’s multiple comparison test). Graph depicts data from three biological experiments.