Fig. 4: Gene specific transcription is altered by loss of CpxA in Y. pseudotuberculosis. | npj Antimicrobials and Resistance

Fig. 4: Gene specific transcription is altered by loss of CpxA in Y. pseudotuberculosis.

From: Cpx-signalling in Yersinia pseudotuberculosis modulates Lipid-A remodelling and resistance to last-resort antimicrobials

Fig. 4

Quantitative RT-PCR was performed on mRNA isolated from Y. pseudotuberculosis strain YPIII07/pIB102 (ΔcpxA full-length null mutant) cultured in LB at 26 °C (A) or at 37 °C (B) until mid to late logarithmic phase (i.e.: an incubation period of 5 h). Data is a collation of four independent experiments composed of three technical replicates in which gene specific transcription of the 12 values was separately normalised against the mean cycle threshold of three independent housekeeping genes, 16S rRNA gene (n = 12), gyrB (n = 12), and rpoC (n = 12). After which all 36 data values are individually represented in a scatter plot as fold change relative to the calibrator control (parental Y. pseudotuberculosis strain YPIII/pIB102 normalised to Log2fold = 0). The median value from all data points is indicated by a blue line, and the actual fold difference in up- (>1.0) or down-regulation (<1.0) of specific gene expression relative to calibrator is indicated in parenthesis. Statistical analysis was performed using a two-tailed, unpaired t-test with Welch’s correction where equal variance is not assumed, and confidence interval was set to 99%. Differences with a P-value of <0.05, < 0.01 or < 0.001 were considered significantly different from parent and are indicated by a red single (*), double (**) or triple (***) asterisk situated immediately above the respective data points on the scatter plot. Analysed genes were: lpxP encoding for Palmitoleoyl acyltransferase (locus tag YPK_0430); lpxL encoding for Lauroyl acyltransferase (YPK_1665); arnB/pmrH/L-ara4N encoding for UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase (YPK_1831); arnF encoding for Undecaprenyl phosphate-alpha-L-ara4N flippase subunit ArnF (YPK_1837); pmrE/ugd encoding for UDP glucose 6-dehydrogenase (YPK_2077); pmrC encoding for serine-type D-Ala-D-Ala carboxypeptidase/endopeptidase (penicillin-binding protein 4) (YPK_3740); pmrA/basR encoding for the two-component response regulator PmrA (YPK_3741).

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