Fig. 2: Sequence polymorphisms in the erm(T) promoter regions of cMLS_B isolates.

The sequence is shown for one isolate with each mutation type (see Supplementary Fig. 2 for all sequences). Polymorphism names are indicated as shown in Table 1. a Multiple sequence alignment. Sequences shown are from the top: iMLS_B isolates, erm(T) regulatory regions on plasmid pRW35¹⁵ and of the emm92 WV-iGAS; cMLS_B isolates, no mutations in the promoter region (Nm), and acquired duplications Dup18 (line 4, IR2’-orange arrow), Dup30 (line 5, IR2’-purple arrow), and Dup79 (line 7, IR’-blue lines); and the deletion Del74 polymorphism (line 6). The ErmTL peptide sequence is shown in blue text, whereas the ErmT protein sequence is shown in black (green for the Dup79 isolate shown in line 7). All Shine Dalgarno sequences are shown by a green box, with the start codon annotated in green text and the erm(T)L stop codon in red text. b Schematic representation of sequence polymorphisms in the erm(T) regulatory region, panel created with BioRender.com. The erm(T)L is shown by the teal line with the stop codon annotated by red asterisks. Inverted repeats are shown as gray boxes. Dotted lines indicate polymorphism sections, with the resulting alternate inverted repeats shown as gray boxes with a dotted outline.