Fig. 3: Effects of functional DNA damage repair in cell free assays. | BJC Reports

Fig. 3: Effects of functional DNA damage repair in cell free assays.

From: Characterizing functional DNA damage and response caused by the combination of CHK1 and WEE1 inhibitors in ovarian and breast cancer models

Fig. 3

Functional capacity of cell lysates to repair DNA damage using the ExSy-SPOT assay; 8oxoG, AbaS, Etheno, Glycols- base excision repair (BER), Cisp- intra strand cross link repair (ISCLR) and Cisp, CPD64-nucleotide excision repair (NER). The Next-SPOT assay studied the ability to repair double strand breaks i.e., homologous recombination (HR), non-homologous end joining (NHEJ), single strand annealing (SSA) and alternative end-joining (ALT-EJ). OVCAR-3 and MDA-MB436 cells were exposed to control, adavosertib and the combination of adavosertib and SRA737. The results have been normalized to the control of each experiment. The experiments were carried out in triplicate and differences between control and treated samples were carried out using pared t tests. Symbol * denotes statistical significance. AbaS Abasic Sites, Etheno = Etheno bases, CPD64 = 6-4 photoproducts, Cisp cisplatin adducts.

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