Fig. 2: Median MSI marker read depth as an assay quality control metric, and the impact of repeat testing poor quality samples. | BJC Reports

Fig. 2: Median MSI marker read depth as an assay quality control metric, and the impact of repeat testing poor quality samples.

From: A novel colorectal cancer test combining microsatellite instability and BRAF/RAS analysis: Clinical validation and impact on Lynch syndrome screening

Fig. 2

a Absolute MSI score compared to median MSI marker read depth of training and validation cohort samples analysed by the Newcastle MSI-Plus Assay. The localised regression lines include shading, coloured by reference MSI status, to represent 95% confidence intervals. The vertical dotted lines represent a median MSI marker read depth of 100 or of 500. b The effect of sample dilution and/or purification on MSI score and median MSI marker read depth of samples that initially failed quality control (median reads per MSI marker <100). The lines connect original sample data to diluted and/or purified sample data.

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