Fig. 1: Fibroblasts predominate in primary human non-small cell lung cancer culture from tissue resections by passage 3. | BJC Reports

Fig. 1: Fibroblasts predominate in primary human non-small cell lung cancer culture from tissue resections by passage 3.

From: Patient derived cancer-associated fibroblasts from non-small cell lung cancer undergo phenotypic drift in culture

Fig. 1

a Protocol for digestion and culture of fibroblasts, with analysis by flow cytometry at different stages of culture; b Representative image of fibroblasts growing in culture, scale bar represents 50 μm; c Proportion of cells in tumour samples which are positive for lineage markers EpCAM, CD45 or CD31 compared to proportion which are fibroblasts demonstrating by passage 3 fibroblasts dominate in culture. Error bars show standard deviation. N = 3.

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