Fig. 3: Changes in CAF subset heterogeneity throughout culture.

a FlowSOM schematic of fibroblasts from tumour and NCL at digest (pink) and passage 3 culture (black) compared to UMAP locations of CAF subsets previously defined in Mathieson et al. for NSCLC; b Location of CAF subsets on a UMAP of the data in this study; c Proportions of each CAF subset passages 0, 1 and 3 for both tumour and non-cancerous lung (NCL). N = 10 P0, N = 4 P1, N = 5 P3. Error bars show standard deviation.