Fig. 5: Functional assessment of fibroblasts derived from tumour and non-cancerous lung at different stages of culture. | BJC Reports

Fig. 5: Functional assessment of fibroblasts derived from tumour and non-cancerous lung at different stages of culture.

From: Patient derived cancer-associated fibroblasts from non-small cell lung cancer undergo phenotypic drift in culture

Fig. 5

a Transwell migration assay (i) Representative images showing fibroblasts that have migrated across the transwell membrane stained with crystal violet for quantification; (ii) Comparison of migratory ability between early and late passage for CAFs and NFs (higher absorbance reading indicates more cells migrated); b Collagen contraction assay (i) Representative images showing comparison of contractile ability of early CAFs and NFs; (ii) Comparison of contractile ability between early and late passage of CAFs and NFs; c Gap closure migration assay (i) Representative images showing the initial gap between fibroblasts (left at 0 h) and after 48 h when the fibroblasts have migrated to close the gap (scale bar 500 μm); (ii) Gap size proportions after 0, 24 and 48 h for CAFs and NFs at late passage. N = 3 independent CAF lines for all assays, statistics shown are uncorrected Fishers LSD for A and B, and Tukey’s multiple comparisons test in C, *p < 0.05, **p < 0.01.

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