Fig. 6: Vascular- and muscle content after seven months.

a A representative area from the PLATE graft zone seven months after surgery including two regions of interest (ROI), Masson’s trichrome. b Collagen IV immunohistochemistry stain in the top ROI used for manual counting of vascular structures (orange arrows) represented as bar charts in absolute numbers (left bar chart) and relative to the area of the graft (right bar chart). c Smooth muscle myosin stain showing smooth muscle tissue within the PLATE graft zone. The smooth muscle area was quantified using machine learning and represented both in absolute values (left bar chart) and relative to the area of the graft zones (right bar chart). Error bars = SEM. *p < 0.05. ns = not significant. All scale bars = 50 μm.