Fig. 2: VEEV-induced SIE over time. | npj Viruses

Fig. 2: VEEV-induced SIE over time.

From: Venezuelan equine encephalitis virus non-structural protein 3 dictates superinfection exclusion in mammalian cells

Fig. 2

a Schematic representation of the experimental approach. Vero cells were transduced with VRP-mCherry (10 VRPs/cell) and, at indicated time points, transduced with VRP-eGFP (10 VRPs/cell). b Twenty-four hours post primary transduction, fluorescent cells were visualized using fluorescence microscopy. c Images were analyzed using a customized CellProfiler pipeline to quantify mCherry-, eGFP-, and dual-positive cells of total fluorescent cells. Bars represent the mean and standard deviation of two independent experiments with each of eight biological replicates. Asterisks indicate significant differences in the ratio of mCherry-, eGFP-, and dual-positive cells compared to 0 h and between the following time intervals (****P < 0.0001, detailed information in Supplementary Table 3).

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