Table 1 Samples sequenced in this study by the multiplex PCR-NGS assay

Sample	Sampling date^a	Location	qPCR (Ct)^b	Accession number^c
51	11/30/2023	Durazno	39.0/28.1	PP747340
70^*	12/01/2023	Paysandú	34.0/27.0	PP747341
95	12/04/2023	San José	40.0/26.0	PP747342
107	12/06/2023	Río Negro	37.3/29.8	PP747343
158^*	12/08/2023	San José	35.6/25.8	PP747344
184	12/11/2023	Paysandú	37.9/31.0	PP747345
191	12/12/2023	Río Negro	38.7/27.3	PP747346
197	12/13/2023	Río Negro	39.5/28.4	PP747347
198	12/13/2023	San José	34.9/24.9	PP747348
199	12/13/2023	Artigas	40.6/31.1	PP747349
227	12/27/2023	Lavalleja	41.1/28.1	PP747350
228	12/27/2023	Lavalleja	38.7/28.5	PP747351
230	12/27/2023	Treinta y Tres	38.5/27.2	PP747352
248	01/15/2024	San José	37.0/28.5	PP747353
250	01/16/2024	Río Negro	31/22.3	PP747354

^*Sequences from samples 70 and 158 were previously available in GenBank (PP620641 and PP620642).
^aAll samples are from equine hosts and obtained from brain tissue or cerebral cortex, except for sample 186, which is from cerebrospinal fluid.
^bThe Ct values obtained using the Lambert et al.³⁴ and Brault et al.³⁶ methodologies are indicated and separated by a bar.
^cAccession numbers of complete coding sequences.

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