Fig. 2: Determination of inhibition cutoff percent. | npj Viruses

Fig. 2: Determination of inhibition cutoff percent.

From: Automated and virus variant-programmable surrogate test qualitatively compares to the gold standard SARS-CoV-2 neutralization assay

Fig. 2

ROC plot showing specificity and sensitivity for (a) WT and (b) Delta RBD. c The percent inhibition for post-vaccination/infection samples vs. WT-RBD (blue circles) or Delta-RBD (gray circles) and a panel of negative controls (white circles) comprised of pre-vaccination sera collected prior to 2019 and from other respiratory infections. The dotted line represents the 27% cutoff. Vaccination samples (n = 103) from the PASS cohort received the two-dose BNT162b2 (Pfizer) series, whereas the infection samples (n = 51 serum samples from 17 individuals at 3 time points) represent individuals positive for SARS-CoV-2 with varied vaccination status from the EPICC cohort. The negative control sample panel (n = 203) comprised pre-vaccination confirmed negative samples (n = 103), sera collected prior to the outbreak of SARS-CoV-2 (n = 50), and samples from other respiratory viruses and human coronaviruses (n = 50): 229E (n = 9), HKU1 (n = 9), NL63 (n = 10), OC43 (n = 8), influenza A (n = 6) and influenza B (n = 8).

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