Table 1 Comparison of neutralization assays
Assay | Acronym | Mechanism | Time | Safety | Performance | Caveat |
|---|---|---|---|---|---|---|
Plaque Reduction Neutralization Test | PRNT | Infectious Virus/Cell Based | 5 days | BSL-3 | Gold standard | Time/specialized containment |
Microneutralization Assay | mVNT | Infectious Virus/Cell Based | 2–4 days | BSL-3 | Comparable to PRNT | Time/specialized containment |
Pseudovirus Neutralization Assay | pVNT | Lentivirus, VSV, or other BSL-2 virus /Cell Based | 2–3 days | BSL-2 | Comparable to PRNT | Time/not the whole virus |
Surrogate Virus Neutralization Test | sVNT | Molecular interaction-based/e.g. ELISA, flow cytometry, bead, or sensor based | 1–4 h | BSL-1 or 2 | Comparable to PRNT | Specialized equipment, not the whole virus |
