Fig. 1: Comparison between the sequences of TOSV-B reverse genetic plasmids and those of TOSV lineage B reference genomes. | npj Viruses

Fig. 1: Comparison between the sequences of TOSV-B reverse genetic plasmids and those of TOSV lineage B reference genomes.

From: Genetic diversity of Toscana virus glycoproteins affects the kinetics of virus entry and the infectivity of newly produced virions

Fig. 1

A Schematic representation of the TOSV antigenome segments: Seg-S, Seg-M, and Seg-L. The location of the viral 5’ and 3’ untranslated regions (UTR) and open reading frame of the structural (N, Gn, Gc and RNA-dependent RNA polymerase [RdRp]) and non-structural (NSs and NSm) viral proteins are indicated. The 14 substitutions identified between the reference sequences of the wt TOSV-B and those of the plasmid developed for the reverse genetics system (indicated as RG plasmid) are presented. Non-synonymous substitutions are in bold and their respective change in amino acid is indicated. B Nucleotide differences between TOSV-B strains. Sequences were aligned using MUSCLE software. Dots indicate identical nucleotides. Note that, for Nice/113 strain, Seg-S sequence is incomplete at the 3’ UTR, thereby preventing comparison at position 1860.

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