Fig. 2: TOSV-A viruses produce higher infectious titers than TOSV-B.

Growth curves of wt TOSV-A, wt TOSV-B, rTOSV-A and rTOSV-B in A549 (A) or A549 Npro (B) cells (MOI = 0.01). Cell supernatants were collected at 24-, 48-, and 72-hours post-infection, and viral titers were obtained by TCID50 limiting-dilution assays in A549V cells. Each experiment was performed in triplicate and at least three times independently. Bars indicate standard deviations. Light grey dashed lines indicate the threshold of virus detection [2.25 Log₁₀(TCID50/mL)]. Statistical significance was determined using multiple Wilcoxon tests with the Benjamini-Yekutieli (BY) p-value adjustment method: p < 0.05 (*) and p < 0.01 (**). Black and grey stars indicate the statistical significance of the rescued viruses compared to their parental virus strain (i.e., wt TOSV-A or wt TOSV-B, respectively).