Fig. 3: Lfcin promotes HAdV-C5 attachment to and entry into human skeletal myoblasts.
From: Lactoferricin enables adenovirus infection of human skeletal muscle cells
a Analysis of HAdV-C5 infection (6000 vp/cell) of myoblasts, Lfcin added at various times during the inoculation procedure; 1), HAdV-C5 infection only; 2), Lfcin added to cells for 30 min, then removed (without washing) before HAdV-C5 inoculation; 3), HAdV-C5 pre-incubated with Lfcin 30 min prior to inoculation on cells; 4), HAdV-C5 and Lfcin inoculated on cells simultaneously; or 5), Lfcin added to medium for 48 h after HAdV-C5 inoculation. Samples were stained for HAdV capsid protein and cell nuclei. b qPCR-based analysis of HAdV binding to myoblasts with or without Lfcin. 5000 vp/cell were incubated with 2 µM Lfcin and added to cells on ice for 30 min. Binding is normalised against GAPDH and is presented as fold change of viral DNA compared to mock-treated cells. Data are from three independent experiments, presented as mean ± SD. Statistical significance was determined with one-way ANOVA; ns, not significant; *** P < 0.001.
