Fig. 5: Transcriptional changes of annexin, 14-3-3ε, and prohibitin following JEV infection. | npj Viruses

Fig. 5: Transcriptional changes of annexin, 14-3-3ε, and prohibitin following JEV infection.

From: Transcriptomic response of mosquitoes to Japanese encephalitis virus and identification of its potential entry factors

Fig. 5

A–C RT-qPCR analysis of RNA extracted from Hsu cells after infection (1 MOI) with JEV at 1 and 2 days post-infection (dpi) using primers for the three genes. The graphs illustrate gene fold changes after infection compared to the mock. D Focus forming assay of media collected from cells used in (A–C), quantifying JEV virions. Kruskal-Wallis with Dunn’s multiple comparisons test was used for A, and One-way ANOVA with Tukey’s multiple comparisons test for (B–D). The error bars indicate the standard error of the mean (SEM) of biological replicates. Asterisks denote significant differences between compared samples. ns, not significant; *p < 0.05; ***p < 0.001; ****p < 0.0001. E–G General linear regression analysis was performed for the three genes and JEV replication on all data points (n = 12). Error bars indicate 95% confidence interval of the linear regression. E–G graphs correspond to (A–C) graphs. The raw data for the qPCR reactions are provided in Supplementary Data 4.

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