Fig. 6: Effect of silencing the potential entry factors of JEV on its replication and antibody neutralisation assay. | npj Viruses

Fig. 6: Effect of silencing the potential entry factors of JEV on its replication and antibody neutralisation assay.

From: Transcriptomic response of mosquitoes to Japanese encephalitis virus and identification of its potential entry factors

Fig. 6

dsRNAs were designed against annexin, 14-3-3ε, and prohibitin and double transfected into Hsu cells. A–C Confirmation of silencing of the three genes using RT-qPCR. D Two days after the second transfection, cells were infected with 1 MOI of JEV and analysed 2 dpi using RT-qPCR. The data presented are combined from two independent experiments. E Antibody neutralisation assay using anti-prohibitin and anti-Flag (control) alongside JEV infection. In Mock+JEV, no antibody was used. RT-qPCR was used to determine the fold changes in the gRNA levels of JEV. Unpaired t-test was used for data analysis in (A–D), and One-way ANOVA with Tukey’s multiple comparisons test was used for (E). The error bars indicate the standard error of the mean (SEM) of biological replicates. Asterisks denote significant differences between compared samples. *p < 0.05; ***p < 0.001; ****p < 0.0001. The raw data for the qPCR reactions are provided in Supplementary Data 4.

Back to article page