Fig. 3: Baseline differences and quantification of free cholesterol in wild-type and STAT1−/− HEp-2 cells. | npj Viruses

Fig. 3: Baseline differences and quantification of free cholesterol in wild-type and STAT1−/− HEp-2 cells.

From: STAT1 signaling controls cholesterol metabolism in epithelial cells and RSV-induced syncytia formation

Fig. 3

A UpSet plot demonstrating differentially expressed genes that are shared and unique to infected wild-type and STAT1−/− HEp-2 after comparison to respective uninfected controls with thresholds p < 0.05 and |Log2 Fold change | ≥ 1. B Cleveland Dot Plot of Gene Set Enrichment Analysis (GSEA) of Gene Ontology comparing processes enriched in both infected wild-type and STAT1−/− HEp-2 cells based on Normalized Enrichment Scores (NES). C Quantification of total free cholesterol by luminescent enzymatic assay in uninfected wild-type (green) and STAT1−/− (red) HEp-2 cells. Statistical analysis: Mann-Whitney test. D Quantification of total free cholesterol by luminescent enzymatic assay in wild-type and STAT1−/− HEp-2 cells, which were uninfected (grey), treated with 5 mM methyl-beta-cyclodextrin (MβCD, orange), infected at MOI 0.05 (light red), infected at MOI (dark red), or treated with 100 ng/ml IFN-α2 and 50 ng/mL IFN-γ at 24 and 48 hpi. Only significant differences between untreated and treated cells within a cell type or within treatments between cell types are shown. Statistical analysis: two-way ANOVA with Fisher’s LSD test. Mean ± SD of four independent experiments (two technical replicates) shown for (C, D).

Back to article page