Fig. 7: Integration of viral fusion protein into host cellular membranes. | npj Viruses

Fig. 7: Integration of viral fusion protein into host cellular membranes.

From: STAT1 signaling controls cholesterol metabolism in epithelial cells and RSV-induced syncytia formation

Fig. 7

Western blot of membrane fractions and total cell lysates in uninfected (‘Mock’) and rRSV-A-0594-eGFP-infected (MOI 0.5) wild-type (A) and STAT1−/− HEp-2 (B) HEp-2 cells at 48 hpi. Infected cells were left untreated, treated with 50 μM Gemfibrozil (GFZ), treated with 5 μM methyl-β-cyclodextrin (MβCD) and 50 μM GFZ, or with 10 μM 25-hydroxycholesterol (25-HC). Membrane fractions were stained for prefusion F protein, postfusion F protein, and Na+-K+-ATPase (loading control). Total cell lysates were stained for total F protein (prefusion and postfusion conformation) and β-Actin (loading control). Images representative of three independent experiments.

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