Fig. 2: Ploidy and arrest of radiation-induced giant cells.

a Flow cytometry analysis of 12 Gy irradiated A549 cells showing two different populations i.e. non-giant and giant cells. b Ploidy estimation using propidium iodide (PI) staining, for both populations. c Cell cycle analysis for both populations. d β-gal staining of senescence in both unirradiated and 12 Gy irradiated A549 cells.