Fig. 3: Organoid growth monitoring in SRH: From undifferentiated organoids to normal-like organoids.
From: In vivo organoid growth monitoring by stimulated Raman histology
a Phase contrast images of the organoid imaged on different days (day 1 (D1), day 4 (D4), day 5 (D5), day 7 (D7) and day 11 (D11)) of culture. b SRH images of the same organoid at D1 acquired at different depths, z step = 5 µm, scale bar = 200 µm, c SRH images of the same organoid at D4 acquired at different, z step = 8 µm, scale bar = 250 µm, d SRH images of the same organoid at D7 acquired at different depths, z step = 20 µm, scale bar = 300 µm, e 3D-reconstruction from the images acquired in b (D1), f 3D-reconstruction from the images acquired in c (D4), g 3D-reconstruction from the images acquired in d (D7). Phase contrast images, scale bar = 200 µm, 3D-reconstructions, scale bar = 100 µm. Total power at the sample plane: 90 mW.
