Fig. 6: 2D/3D HiLoTRUST images of human lung cancer specimens.
From: Rapid full-color serial sectioning tomography with speckle illumination and ultraviolet excitation
a Cross-sectional imaging of the first specimen using HiLoTRUST (top left) and TRUST (bottom right). The inset at the bottom left shows a photograph of the specimen. b Zoomed-in TRUST image (left) and HiLoTRUST image (right) of the region in (a) marked with a solid orange box. c Another cross-section of the first specimen imaged with HiLoTRUST (top left) and TRUST (bottom right). d Zoomed-in TRUST image and HiLoTRUST image of the region in c marked with a solid green box. e 3D TRUST image of the first specimen. f Zoomed-in 3D TRUST image of the region marked by a solid orange box in e. g 3D HiLoTRUST image of the first specimen. h Zoomed-in 3D HiLoTRUST image of the region marked by a solid green box in g. i Zoomed-in 3D HiLoTRUST image of the region marked by a solid red box in h. j Cross-sectional imaging with HiLoTRUST (top left) and TRUST (bottom right) of the second specimen. The inset at the bottom left shows a photograph of the specimen. k Zoomed-in TRUST image (left) and HiLoTRUST image (right) of the region in j marked with a solid orange box. l 3D distribution of the desmoplastic stroma extracted from the reconstructed 3D model in m utilizing color difference. n A magnified image of the solid white box region in (l) overlaid with nuclei within cells (predominantly cancer cells). This visualization provides an approximation of the 3D distribution relationship between collagen fibers and cancer cells. Note: TRUST and HiLoTRUST images have been normalized with min-max normalization for better visualization.
