Fig. 10: High-speed PAM for histology.

a Label-free UV-PAM virtual histology of undecalcified bone via unsupervised deep learning (top) and the corresponding H&E histology images (bottom). Close-up images show neoplastic spindle cells (red arrows) arranged in vague streaming and fascicular patterns (i), and a nodule of neoplastic chondroid material (ii, yellow circle), with corresponding H&E histology images. Scale bars, 500 µm (a–d), 100 µm (in all closed-up images) (adapted with permission from ref. ¹⁰³). b Image gallery of an unstained mouse brain embedded in agarose blocks. All features are shown in coronal view, highlighting the absorption contrast by DNA/RNA (adapted with permission from ref. ¹¹⁰).