Fig. 9: High speed PAM in dermatology.
a PAM images of healthy skin (right) versus adjacent psoriatic skin (left). The psoriatic skin image shows the top part of elongated capillary loops (blue arrow) that almost climbed to the skin surface through elongated rete ridges. The capillaries appeared in green, interleaved with thickened epidermal (EP) structures as a result of acanthosis. These structures appeared in red with poor contrast due to loss of pigmentation. Below the epidermis, a dilated and dense vascular structure of the dermis (DR) is resolved (in red). Capillary loops were observed in green and separated from the epidermis (red) and the dilated dermal vasculature (red). Scale bars, 200 μm. Adjacent healthy skin showed a layered EP with clearly resolved vessels in the DR. Scale bars, 200 μm (adapted with permission from ref. 96). b Top- (top) and side- (bottom) view PAM images of a subcutaneously inoculated B16-melanoma in a nude mouse (adapted with permission from ref. 100). c High-speed PAM of blood perfusion in a finger during brachial cuffing and after release along with corresponding depth-encoded images. All scale bars are 200 μm (adapted with permission from ref. 102).
