Fig. 2: Post-ischemic inflammation.
From: Interventional real-time molecular MRI for targeting early myocardial injury in a pig model
A Flow cytometry characterization of innate immune cells in the blood after I/R. Above FACS plots indicate the gating scheme for neutrophils and monocyte subsets. Red boxes indicate final cell populations, while black boxes were used for further subtype characterization. Below Timeline analysis of innate immune cells from baseline to 4 h after onset of ischemia for neutrophils, classical-typed CD14+CD163neg monocytes and nonclassical-typed CD14negC163+ monocytes. *p < 0.05 for the comparison of the time point at 3 h versus baseline; n = 3 per group, Welch’s unpaired t test. B Immunofluorescence staining of cardiac tissue from “Lesion” and “Remote” targeting P-selectin expression (red) and nuclei (dapi-blue). After injury, P-selectin expression is significantly increased in the “Lesion” area compared to “Remote”; n = 6 per group, p = 0.02. Yellow arrows highlight examples of positive P-selectin or DAPI staining. *p < 0.05; Mann-Whitney U test.
