Fig. 3: Targeting P-selectin in vitro.
From: Interventional real-time molecular MRI for targeting early myocardial injury in a pig model
Binding of functionalized MPIO P-selectin (Psel-MPIO) to platelets and endothelial cells was evaluated using an incubation assay for endothelial cells (n = 60 per group, above) or an in vitro flow chamber model for platelets (n = 6 for P-selectin MPIO versus n = 3 control MPIO, below) in vitro. For comparison we used functionalized MPIO targeting unspecific IgG (control MPIO). Both essays confirmed enhanced binding of P-selectin MPIO to platelets (p < 0.0001) or endothelial cells (p < 0.0001) in comparison to control MPIO. Yellow arrows indicate MPIO. *p < 0.05; Mann–Whitney U test.
