Fig. 3: Approaches to monitor specific aspects of neuronal and glial cell activity in vivo and in vitro.
From: The regulation of reporter transgene expression for diverse biological imaging applications
a Viral tools can be used to transduce neurons in the anterograde (from soma to axon terminal) or retrograde direction (from axon terminal back to soma) to map inputs and outputs of select neural circuits. b A whole suite of transgenic tools has been developed to monitor intracellular calcium, potassium, sodium, zinc, ATP, pH, membrane voltage, and neurotransmitter and neuromodulator concentrations. c These tools can be targeted to specific neuronal or glial cell populations via the use of different promoter sequences, or to different sub-cellular compartments or organelles by adding a specific peptide targeting sequence to the transgene (e.g. NLS). (Created in BioRender. Battison, A. (2024) https://BioRender.com/f08h833).
